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Used to formulate CAPS buffer, a zwitterionic buffer useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electro transfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. and minimal reactivity with enzymes or proteins, minimal salt effects.
Jelena Zaitseva.; I Barry Holland.; Lutz Schmitt. The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli.Acta Crystallogr D Biol Crystallogr.2004,60(6), 1076-1084.
Daniele de Sanctis.; Sylvia Dewilde.; Clemens Vonrhein.; Alessandra Pesce.; Luc Moens.; Paolo Ascenzi.; Thomas Hankeln.; Thorsten Burmester.; Marco Ponassi.; Marco Nardini.; Martino Bolognesi. Bishistidyl heme hexacoordination, a key structural property in Drosophila melanogaster hemoglobin.J Biol Chem.2005,280,(29), 27222-27229.
Buffer, pKa = 10.40 at 20o: Biochemistry, 5, 467 (1966).
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant