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CHES is zwitterionic buffer useful in pH range 8.6 to 10.0.CHES is widely utilized as a buffer which is used for investigations on pH-dependent processes in enzymology. It has been shown to have an unusually high affinity for the iodoacetate binding site of liver alcohol dehydrogenase.
Biological buffer, pKa = 9.3 at 20o: Biochemistry, 5, 467 (1966).
Ferreira, C. M. H.; Pinto, I. S. S.; Soares, E. V.; Soares, H. M. V. M. (Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions - a review. RSC Adv. 2015, 5 (39), 30989-31003.
Devi, T. S. R.; kumar, J. S.; Ramkumaar, G. R. DFT analysis on the molecular structure, vibrational and electronic spectra of 2-(cyclohexylamino)ethanesulfonic acid. Spectrochim. Acta, Part A 2015, 137, 761-777.