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115-39-9 - Bromophenol Blue - Bromphenol Blue - A18469 - Alfa Aesar

A18469 Bromophenol Blue

CAS Number
115-39-9
Synonyms
Bromphenol Blue

Size Price ($) Quantity Availability
10g 28.20
50g 73.10
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Bromophenol Blue

MDL
MFCD00005875
EINECS
204-086-2

Chemical Properties

Formula
C19H10Br4O5S
Formula Weight
669.98
Melting point
ca 270° dec.
Solubility
Freely soluble in NaOH. More soluble in methyl and ethyl alcohols ,benzene, and in acetic acid. Slightly soluble in water (0.4g/100g) at 20°C. Soluble in methanol (10 mg/mL.).

Applications

It is employed as an electrophoresis tracking dye and also finds its uses as an industrial dye, a laboratory acid-base indicator and a biological stain to detect proteins and nucleic acids (absorbance at 610 nm), particularly when staining living tissues. In gel electrophoresis it can be used as a color marker and as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.

Notes

Transition interval: pH 3.0 (yellow) to pH 4.6 (blue)

Literature References

Menzies DW. Bromphenol blue as a stain for muscle striations.Stain Technol.1961,36285-287.

Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue.Anal Biochem.,1978,88(2), 605-11.

Other References

Merck
14,1444
Beilstein
61698
Harmonized Tariff Code
2934.99
TSCA
Yes
RTECS
SJ7453000

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