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72-57-1 - Trypan Blue, dye content >60% - C.I. 23850 - Direct Blue 14 - A18600 - Alfa Aesar

A18600 Trypan Blue, dye content >60%

CAS Number
72-57-1
Synonyms
C.I. 23850
Direct Blue 14

Size Price ($) Quantity Availability
25g 26.82
100g 70.47
500g 238.40
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Trypan Blue, dye content >60%

MDL
MFCD00003969
EINECS
200-786-7

Chemical Properties

Formula
C34H24N6Na4O14S4
Formula Weight
960.82
Melting point
>300°
Solubility
Soluble in water (10 mg/ml at 25°C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).

Applications

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

Notes

Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Literature References

McMahon TA.; Rohr JR. Trypan blue dye is an effective and inexpensive way to determine the viability of Batrachochytrium dendrobatidis zoospores.EcoHealth2014,11(2), 164-167.

Serafini R.; Longobardi V.; Spadetta M.; Neri D.; Ariota B.; Gasparrini B.; Di Palo R. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates.Reproduction in Domestic Animals = Zuchthygiene,2014,49(1), 41-47.

GHS Hazard and Precautionary Statements

Hazard Statements: H350

May cause cancer.

Precautionary Statements: P201-P281-P202-P308+P313-P405-P501a

Obtain special instructions before use. Use personal protective equipment as required. Do not handle until all safety precautions have been read and understood. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,9792
Harmonized Tariff Code
3204.14
TSCA
Yes
RTECS
QJ6475000

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