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Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
McMahon TA.; Rohr JR. Trypan blue dye is an effective and inexpensive way to determine the viability of Batrachochytrium dendrobatidis zoospores.EcoHealth2014,11(2), 164-167.
Serafini R.; Longobardi V.; Spadetta M.; Neri D.; Ariota B.; Gasparrini B.; Di Palo R. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates.Reproduction in Domestic Animals = Zuchthygiene,2014,49(1), 41-47.
Hazard Statements: H350
May cause cancer.
Precautionary Statements: P201-P202-P281-P308+P313-P501c
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Use personal protective equipment as required. IF exposed or concerned: Get medical advice/attention. Dispose of contents/ container to an approved waste disposal plant