Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

7240-90-6 - 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+% - X-gal - B21034 - Alfa Aesar

B21034 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

CAS Number
7240-90-6
Synonyms
X-gal

Size Price ($) Quantity Availability
10mg 26.10
50mg 53.10
250mg 171.00
Add to Cart Add to Quote Request View Item

5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

MDL
MFCD00005666
EINECS
230-640-8

Chemical Properties

Formula
C14H15BrClNO6
Formula Weight
408.64
Melting point
ca 236° dec.
Sensitivity
Moisture & Light Sensitive
Solubility
Soluble in 100 mM in DMSO.

Applications

It is a substrate for beta-galactosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It is also used to detect activity of this enzyme in histochemistry and bacteriology. It is employed as a colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds. They are used in biochemical assays and in diagnosis as indicators, particularly in the form of enzyme substrates. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Notes

Moisture & Light sensitive. Store away from oxidizing agents, heat, water/moisture and light. Store under inert gas. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Literature References

Christiaen L, et al. X-gal staining of electroporated sea squirt (Ciona) embryos.Cold Spring Harbor protocols.20092009(12), prot5346.

Kim MY, et al. X-gal inhibits the swarming of Vibrio species.J. Microbiol. Methods.200666(3), 552-555.

Other References

Beilstein
1402009
Harmonized Tariff Code
2934.99
TSCA
No

Recommended

Recently Viewed

Chemicals

Life Science

Metals & Materials

Catalysts

Analytical & Labware