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Used as a pharmaceutical intermediate to reduce toxicity and as an enzyme substrate in diagnostic reagents. Salmon-gal is used in conjunction with IPTG for detection of β-galactosidase activity in bacterial colonies in a colometric assay to detect recombinants (white) from the non-recombinants (SALMON). In conjunction with X-glu, salmon-gal is useful for simultaneous detection of GUS and Lac activities on the same plate.
Simonson MS, et al. Rapid characterization of growth-arrest genes in transient transfection assays.BioTechniques.199518(3), 434-6, 438, 440-2.
Aimin Liu, et al. Alteration of limb and brain patterning in early mouse embryos by ultrasound-guided injection of Shh-expressing cells.Mechanisms of Development.199875(1-2), 107-115.