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It is a fluorogenic substrate for measuring of ß-glucoronidiase activity, for rapid sensitive identification of Escherichia coli. MUG is a substrate for glucuronidase. It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
Farnleitner AH, et al. Hydrolysis of 4-methylumbelliferyl-beta-D-glucuronide in differing sample fractions of river waters and its implication for the detection of fecal pollution.Water Res.200236(4), 975-81.
Ekholm DF and Hirshfield IN. Rapid methods to enumerate Escherichia coli in foods using 4-methylumbelliferyl-beta-D-glucuronide.J AOAC Int.200184(2), 407-15.