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B21674 Safranine O

CAS Number
477-73-6
Synonyms
C.I. 50240
3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride

Stock No. Size Price ($) Quantity Availability
B21674-09 10g 23.30
B21674-18 50g 57.70
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Safranine O

MDL
MFCD00011759
EINECS
207-518-8

Chemical Properties

Formula
C20H19ClN4
Formula Weight
350.85
Storage & Sensitivity
Ambient temperatures.
Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Applications

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Notes

Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Literature References

Krings M. The use of biological stains in the analysis of late Paleozoic pteridosperm cuticles.Rev Palaeobot Palynol.2000108(3-4), 143-150.

Rosenberg L. Chemical basis for the histological use of safranin O in the study of articular cartilage.J Bone Joint Surg Am.197153(1), 69-82.

Biological stain.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H318

Causes skin irritation. Causes serious eye damage.

Precautionary Statements: P264b-P280-P302+P352-P305+P351+P338-P310-P332+P313-P362

Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. If skin irritation occurs: Get medical advice/attention. Take off contaminated clothing and wash before reuse.

Other References

Beilstein
3924099
Harmonized Tariff Code
2933.99
TSCA
Yes
RTECS
SG1623000

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