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Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used in immunology for detection of alkaline phosphatase. In addition to this, it acts as an oxidant.
Substrate for determination of various enzymes.
Materska, M.; Konopacka, M.; Rogoliński, J.; Ślosarek, K. Antioxidant activity and protective effects against oxidative damage of human cells induced by X-radiation of phenolic glycosides isolated from pepper fruits Capsicum annuum L. Food Chem. 2015, 168, 546-553.
Xiong, B.; Xu, R.; Zhou, R.; He, Y.; Yeung, E. S. Preventing UV induced cell damage by scavenging reactive oxygen species with enzyme-mimic Au-Pt nanocomposites. Talanta 2014, 120, 262-267.
Hazard Statements: H302-H315-H319-H335
Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P280a-P305+P351+P338-P304+P340-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.