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596-09-8 - Fluorescein diacetate, 97% - B24466 - Alfa Aesar

B24466 Fluorescein diacetate, 97%

CAS Number
596-09-8
Synonyms

Size Price ($) Quantity Availability
5g 43.67
25g 125.66
100g 305.91
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Fluorescein diacetate, 97%

MDL
MFCD00005062
EINECS
209-877-6

Chemical Properties

Formula
C24H16O7
Formula Weight
416.39
Melting point
200-203°
Solubility
Soluble in chloroform, DMSO, ethanol, and water.

Applications

Fluorescein diacetate stains living cells green, and is a substrate for esterases. It is suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes. It is used in studies on intracellular interactions and membrane permeability.

Notes

Store at -20°C. Store in a cool, dry place in tightly closed container. Store away from oxidizing agents.

Literature References

J. Heslop-Harrison & Y. Heslop-Harrison. Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein Diacetate. Stain Technology. 1970, 45 (3), 115-120.

Jack M. Widholm. The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells. Stain Technology. 1972, 47 (4), 189-194.

Other References

Beilstein
62575
Harmonized Tariff Code
3204.90
TSCA
Yes

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