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It is used as substitute for the carcinogenic benzidine in the chromogenic assay of peroxidases.
Shin-ichi Kaiho.; Koji Mizuno. Sensitive assay systems for detection of hemoglobin with 2,7-diaminofluorene: Histochemistry and colorimetry for erythrodifferentiation. Anal. Biochem. 1985, 149 (1),117-120.
S.J. Gebran.; E.L. Romano, H.A. Pons, L. Cariani.; A.N. Soyano. A modified colorimetric method for the measurement of phagocytosis and antibody-dependent cell cytotoxicity using 2,7-diaminofluorene. Journal of Immunological Methods. 1992, 151 (1-2),255-260.
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c
Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant