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N-Acetyl-D-alanine may be used with other D-aminoacylated amino acids as a substrate for the identification, differentiation and characterization of D-aminoacylase(s)/amidohydrolase(s). N-acetyl-D-alanine may be used to study aglycon pocket specific binding on vancomycin.
P J Loll, R Miller; C M Weeks; P H Axelsen. A ligand-mediated dimerization mode for vancomycin. Chemistry & Biology. 1998, 5(5), 293-298.
Yasushi Nitanai; Takanori Kikuchi; Kouji Kakoi; Shinji Hanamaki; Ikuhide Fujisawa; Katsuyuki Aoki; Yasushi Nitanai; Takanori Kikuchi; Kouji Kakoi; Shinji Hanamaki; Ikuhide Fujisawa, Katsuyuki Aoki. Crystal structures of the complexes between vancomycin and cell-wall precursor analogs. Journal of Molecular Biology. 2009, 385(5), 1422-1432.