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Bovine Serum Albumin is used extensively by molecular biologists to stabilize labile enzymes such as restriction endonucleases, polymerases, and ligases.
Many nucleases are irreversibly inactivated by acetylation, but the stabilizing action of BSA is unaffected by this procedure.(1,2) Thus, acetylation ensures that the use of albumin does not introduce additional nucleases to reaction mixture.
1. Epstein, C.J. and Goldberger, R.F. J. Biol. Chem. 1964, 239, 1087-1089.
2. Lackey, D. And Linn, S. Meth. in Enzymol. 1980, 65, 26-28.