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J19535 p-Nitro Blue Tetrazolium chloride (NBT), Ultrapure, Affymetrix/USB

CAS Number
298-83-9
Synonyms
5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)tetrazolium dichloride

Stock No. Size Price ($) Quantity Availability
J19535-MD 250mg 34.30
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p-Nitro Blue Tetrazolium chloride (NBT), Ultrapure, Affymetrix/USB

MDL
MFCD00012159
EINECS
206-067-4

Chemical Properties

Formula
C40H30Cl2N10O6
Formula Weight
817.64
Form
Crystalline powder
Melting point
ca 190° dec.
Storage & Sensitivity
Light Sensitive. Ambient temperatures.

Applications

Used with  to detect antigen-antibody alkaline phosphatase complexes. The NBT/BCIP mix is converted to a dense blue compound and will develop at the site of antigen-antibody complexes.

Notes

NBT Stock Solution: 50 mg/ml in 70% dimethylformamide.

BCIP/NBT Developing Solution:

  • 33 ml NBT (50 mg/ml)
  • 5 ml AP Buffer (100mM Tris-HCl, pH 9.5, 100mM NaCl, 5mM MgCl2)
  • Add 16.8 ml BCIP stock (50 mg/ml in 100% dH20)
  • Protect from light and use within 1 hr

GHS Hazard and Precautionary Statements

Hazard Statements: H302-H315-H319-H335

Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Rinse mouth. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Beilstein
4115923
Harmonized Tariff Code
2933.99
TSCA
Yes
RTECS
XF8045000

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