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J19972 Polyethylene Glycol 6000, flakes, Ultrapure, Thermo Scientific

CAS Number
25322-68-3
Synonyms
PEG 6000

Stock No. Size Price ($) Quantity Availability
J19972-A1 1kg 70.50
J19972-A7 5kg 287.00
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Polyethylene Glycol 6000, flakes, Ultrapure, Thermo Scientific

MDL
MFCD01779614
EINECS
500-038-2

Chemical Properties

Formula Weight
5400-6600
Form
Flakes
Melting point
60-63°
Storage & Sensitivity
Ambient temperatures.

Applications

  • Differential precipitation of DNA.
  • Enhances hybridization rate of nucleic acids.
  • Improves efficiency of end-labeling with T4 Polynucleotide Kinase(1,2).
  • Used in ligation of blunt ended DNA.
  • Precipitates bacteriophage ± particles and plasmid DNA.
  • Used in preparing single-stranded M13 DNA.
  • Promotes cell fusion.

Literature References

PEG, with magnesium, causes DNA to undergo a “psi” transition and it collapses into a highly condensed state(1). This results in macromolecular crowding(2) and increased efficiency in end-labeling.

1. Lerman, L.S. Proc Natl. Acad Sci. 1971, 68, 1886.

2. Harrison, B. and Zimmerman, S. B., Anal Biochem. 1986, 158, 307.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H320

Causes skin irritation. Causes eye irritation.

Precautionary Statements: P264b-P280-P302+P352-P305+P351+P338-P332+P313-P362-P501c

Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If skin irritation occurs: Get medical advice/attention. Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Merck
14,7568
Harmonized Tariff Code
3907.20
TSCA
Yes
RTECS
TQ3630000

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