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Laemmli SDS sample buffer, reducing (4X) - J60015 - Alfa Aesar

J60015 Laemmli SDS sample buffer, reducing (4X)

CAS Number
Synonyms

Size Price ($) Quantity Availability
25ml 40.17
50ml 64.27
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Laemmli SDS sample buffer, reducing (4X)

Chemical Properties

Form
Liquid
Solubility
Miscible with water.

Applications

Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins.

Notes

Contains 250mM Tris-HCl(pH 6.8), 8% SDS, 40% glycerol, 8% beta-mercaptoethanol, and 0.02% bromophenol blue.Store in a cool place. Incompatible with oxidizing agents.

Literature References

Lazar, E.; Peterfi, Z.; Sirokmany, G.; Kovacs, H. A.; Klement, E.; Medzihradszky, K. F.; Geiszt, M. Structure-function analysis of peroxidasin provides insight into the mechanism of collagen IV crosslinking. Free Radical Biol. Med. 2015, 83, 273-282.

Saul, J.; Petritis, B.; Sau, S.; Rauf, F.; Gaskin, M.; Reynolds, B. O.; Mineyev, I.; Magee, M.; Chaput, J.; Qiu, J.; LaBaer, J. Development of a full-length human protein production pipeline. Protein Sci. 2014, 23 (8), 1123-1135.

GHS Hazard and Precautionary Statements

Hazard Statements: H318-H315-H312-H303-H412

Causes serious eye damage. Causes skin irritation. Harmful in contact with skin. May be harmful if swallowed. Harmful to aquatic life with long lasting effects.

Precautionary Statements: P273-P280-P305+P351+P338-P310-P362-P312-P363-P321-P322-P501a

Avoid release to the environment. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Wash contaminated clothing before reuse. Specific treatment (see label). Specific measures (see label). Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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