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Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins.
Lazar, E.; Peterfi, Z.; Sirokmany, G.; Kovacs, H. A.; Klement, E.; Medzihradszky, K. F.; Geiszt, M. Structure-function analysis of peroxidasin provides insight into the mechanism of collagen IV crosslinking. Free Radical Biol. Med. 2015, 83, 273-282.
Saul, J.; Petritis, B.; Sau, S.; Rauf, F.; Gaskin, M.; Reynolds, B. O.; Mineyev, I.; Magee, M.; Chaput, J.; Qiu, J.; LaBaer, J. Development of a full-length human protein production pipeline. Protein Sci. 2014, 23 (8), 1123-1135.
Hazard Statements: H227-H302+H332-H311-H317-H318-H335
Combustible liquid. Harmful if swallowed. Harmful if inhaled. Toxic in contact with skin. May cause an allergic skin reaction. Causes serious eye damage. May cause respiratory irritation.
Precautionary Statements: P210-P264b-P270-P271-P272-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P310-P312-P330-P333+P313-P361-P363-P370+P378q-P501c
Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Rinse mouth. If skin irritation or rash occurs: Get medical advice/attention. Remove/Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. In case of fire: Use CO2, dry chemical, or foam Dispose of contents/ container to an approved waste disposal plant