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J60015 Laemmli SDS sample buffer, reducing (4X)

CAS Number

Stock No. Size Price ($) Quantity Availability
J60015-AC 25ml 45.40
J60015-AD 50ml 72.50
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Laemmli SDS sample buffer, reducing (4X)

Chemical Properties

Storage & Sensitivity
Ambient temperatures.
Miscible with water.


Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins.


Contains 250mM Tris-HCl(pH 6.8), 8% SDS, 40% glycerol, 8% beta-mercaptoethanol, and 0.02% bromophenol blue.Store in a cool place. Incompatible with oxidizing agents.

Literature References

Lazar, E.; Peterfi, Z.; Sirokmany, G.; Kovacs, H. A.; Klement, E.; Medzihradszky, K. F.; Geiszt, M. Structure-function analysis of peroxidasin provides insight into the mechanism of collagen IV crosslinking. Free Radical Biol. Med. 2015, 83, 273-282.

Saul, J.; Petritis, B.; Sau, S.; Rauf, F.; Gaskin, M.; Reynolds, B. O.; Mineyev, I.; Magee, M.; Chaput, J.; Qiu, J.; LaBaer, J. Development of a full-length human protein production pipeline. Protein Sci. 2014, 23 (8), 1123-1135.

GHS Hazard and Precautionary Statements

Hazard Statements: H227-H302+H332-H311-H317-H318-H335

Combustible liquid. Harmful if swallowed. Harmful if inhaled. Toxic in contact with skin. May cause an allergic skin reaction. Causes serious eye damage. May cause respiratory irritation.

Precautionary Statements: P210-P264b-P270-P271-P272-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P310-P312-P330-P333+P313-P361-P363-P370+P378q-P501c

Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Rinse mouth. If skin irritation or rash occurs: Get medical advice/attention. Remove/Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. In case of fire: Use CO2, dry chemical, or foam Dispose of contents/ container to an approved waste disposal plant

Other References

Harmonized Tariff Code


  • A10232

    trans-Stilbene, 98%
  • A13004

    tert-Butyl chloride, 98+%
  • J63615

    Laemmli SDS sample buffer, non-reducing (4X)
  • J63139

    Ponceau S, 0.2% v/v soln. in 5% acetic acid
  • J61337

    Laemmli SDS sample buffer, reducing (6X)

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