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J60027 Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade

CAS Number
DNase I

Stock No. Size Price ($) Quantity Availability
J60027-!R 100units 62.90
J60027-!U 500units 158.00
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Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade


Chemical Properties

Liquid, 31 kDa
Storage & Sensitivity
Store at -20°C.
Soluble in 0.15 M sodium chloride (5.0mg/L) clear.


Endonuclease that splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide and removal of DNA from protein samples. Also used in is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines.


One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0. Minimum 2,000 units per ml. Solution at 1 mg/ml containing 50% glycerol and 1 mM calcium chloride.

Literature References

David B N Lee; Nora Jamgotchian; Suni G Allen; Frederick W K Kan; Irene L Hale. Annexin A2 heterotetramer: role in tight junction assembly. American Journal of Physiology - Renal Physiology. 2004 287(3), F481-F491.

Y Shirako; J H Strauss. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis. Journal of Virology. 1994 68(3), 1874-1885.

GHS Hazard and Precautionary Statements

Hazard Statements: H317-H334

May cause an allergic skin reaction. May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statements: P261-P272-P280g-P285-P302+P352-P304+P341-P333+P313-P342+P311-P363-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves. In case of inadequate ventilation wear respiratory protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If skin irritation or rash occurs: Get medical advice/attention. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant

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