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9003-98-9 - Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade - EC - DNase I - J60027 - Alfa Aesar

J60027 Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade

CAS Number
DNase I

Size Price ($) Quantity Availability
100units 61.10
500units 153.00
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Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade


Chemical Properties

Liquid, 31 kDa
Soluble in 0.15 M sodium chloride (5.0mg/L) clear.


Endonuclease that splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide and removal of DNA from protein samples. Also used in is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines.


One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0. Minimum 2,000 units per ml. Solution at 1 mg/ml containing 50% glycerol and 1 mM calcium chloride.

Literature References

David B N Lee; Nora Jamgotchian; Suni G Allen; Frederick W K Kan; Irene L Hale. Annexin A2 heterotetramer: role in tight junction assembly. American Journal of Physiology - Renal Physiology. 2004 287(3), F481-F491.

Y Shirako; J H Strauss. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis. Journal of Virology. 1994 68(3), 1874-1885.

GHS Hazard and Precautionary Statements

Precautionary Statements: P260u-P201-P280-P303+P361+P353-P305+P351+P338-P301+P330+P331-P304+P340-P310a-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

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