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9003-98-9 - Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade - EC 3.1.21.1 - DNase I - J60027 - Alfa Aesar

J60027 Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade

CAS Number
9003-98-9
Synonyms
EC 3.1.21.1
DNase I

Size Price ($) Quantity Availability
100units 61.70
500units 120.00
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Deoxyribonuclease I, bovine pancreas, Molecular Biology Grade

MDL
MFCD00130918
EINECS
232-667-0

Chemical Properties

Form
Liquid, 31 kDa
Solubility
Soluble in 0.15 M sodium chloride (5.0mg/L) clear.

Applications

Endonuclease that splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide and removal of DNA from protein samples. Also used in is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines.

Notes

One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0. Minimum 2,000 units per ml. Solution at 1 mg/ml containing 50% glycerol and 1 mM calcium chloride.

Literature References

David B N Lee; Nora Jamgotchian; Suni G Allen; Frederick W K Kan; Irene L Hale. Annexin A2 heterotetramer: role in tight junction assembly. American Journal of Physiology - Renal Physiology.2004287(3), F481-F491.

Y Shirako; J H Strauss. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis. Journal of Virology,.199468(3), 1874-1885.

Other References

Merck
14,2906
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
RF0750000

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