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BSA blocking buffer, 5% in TBS, with 0.05% Tween-20 is used for saturating excess protein-binding sites on the membranes and microplates in immunoassays.
Krumpel, M.; Reithmeier, A.; Senge, T.; Baeumler, T. A.; Frank, M.; Nyholm, P. G.; Ek-Rylander, B.; Andersson, G. The small chemical enzyme inhibitor 5-phenylnicotinic acid/CD13 inhibits cell migration and invasion of tartrate-resistant acid phosphatase/ACP5-overexpressing MDA-MB-231 breast cancer cells. Exp. Cell. Res. 2015, 339 (1), 154-162.
Ríos-Marco, P.; Ríos, A.; Jiménez-López, J. M.; Carrasco, M. P.; Marco, C. Cholesterol homeostasis and autophagic flux in perifosine-treated human hepatoblastoma HepG2 and glioblastoma U-87 MG cell lines. Biochem. Pharmacol. 2015, 96 (1), 10-19.