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Alcian Blue 8GX is widely utilized as a heteroglycan stain for acidic, neutral, sulfated and phosphate polysaccharides and glycosaminoglycans in tissues. It finds application in both light and electron microscopy. It serves as an adhesive to stick glycol methacrylate sections to glass slides. Due to its large aromatic surface, it is used as a coating agent and modifies the property on the surfaces. It acts as a gelling agent for lubricating fluids and is also used in the modification of carbon paste electrode which promotes redox behavior and stability of electrode.
Kameyama , A.; Dong, W.; Matsuno, Y. Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes. Methods Mol. Biol. 2015, 1314, 325-331.
Candiano, G.; Santucci, L.; Petretto, A.; Inglese, E.; Bruschi, M.; Ghiggeri, G. E.; Boschetti, E.; Righetti, G. Widening and Diversifying the Proteome Capture by Combinatorial Peptide Ligand Libraries via Alcian Blue Dye Binding. Anal. Chem. 2015, 87 (9), 4814-4820