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6132-04-3 - Citrate, 0.5M buffer soln., pH 5.0 - J60125 - Alfa Aesar

J60125 Citrate, 0.5M buffer soln., pH 5.0

CAS Number

Size Price ($) Quantity Availability
250ml 56.24
500ml 88.37
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Citrate, 0.5M buffer soln., pH 5.0


Chemical Properties

It is soluble in water (29.4 g/L) at 20°C. Insoluble in ethanol.


Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity and as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.


Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Literature References

YP Lee.; T Takahashi. An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochemistry. 1972, 14,(1),  71-77.

XZ Shu.; KJ Zhu.; W Song. Novel pH-sensitive citrate cross-linked chitosan film for drug controlled release. International Journal of Pharmaceutics. 2001, 212,(1), 19-28.

GHS Hazard and Precautionary Statements

Hazard Statements: H318

Causes serious eye damage.

Precautionary Statements: P280-P305+P351+P338-P310

Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician.

Other References

Harmonized Tariff Code


  • A10237

    2,2',4,4'-Tetrahydroxybenzophenone, 98+%
  • A12646

    Triethylamine, 99%
  • 12373

    Cadmium chloride hemipentahydrate, ACS, 79.5-81.0%
  • J60408

    Sodium bicarbonate, 1M buffer soln., pH 8.5
  • J61229

    Carbonate, 0.5M buffer soln., pH 10.0

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