Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into F(ab')2 or Fab fragments. Ficin acts on bonds involving uncharged and/or aromatic amino acids. The immobilization of ficin enhances stability against denaturation, heat and autolysis. Immobilization also eliminates any potential for antibody-enzyme adducts that cause continued sample digestion. In addition to these advantages, immobilization allows the ficin to be reused.
M Mariani; M Camagna; L Tarditi; E Seccamani. A new enzymatic method to obtain high-yield F(ab)2 suitable for clinical use from mouse IgGl. Molecular Immunology, 1991, 28(1-2), 69-77.
Filippova IYu; E N Lysogorskaya; E S Oksenoit; G N Rudenskaya; V M Stepanov. L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide--a chromogenic substrate for thiol proteinase assay. Analytical Biochemistry, 1984,143,(2), 293-297.
Hazard Statements: H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Precautionary Statements: P284-P261-P280a-P305+P351+P338-P405-P501a
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.