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Tween 20 blocking buffer solution is used as a washing agent in immunoassays, such as Western blots and ELISAs. It helps to prevent non-specific antibody binding. The buffer is used for washes between each immunoreactions, to remove unbound immunologicals, and eventually for incubation solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
JL Pace.; PJ Morales.; JS Hunt.; TA Phillips. Analysis of the soluble isoforms of HLA-G. Placenta and Trophoblast. 1980, 122 181-203.
M Szı?lagyi.; VR Rivera.; D Neal.; GA Merrill.; MA Poli. Development of sensitive colorimetric capture elisas for Clostridium botulinum neurotoxin serotypes A and B. Toxicon. 2000, 38(3), 381-389.