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615-28-1 - o-Phenylenediamine dihydrochloride, 98+% - 1,2-Diaminobenzene dihydrochloride - J60354 - Alfa Aesar

J60354 o-Phenylenediamine dihydrochloride, 98+%

CAS Number
615-28-1
Synonyms
1,2-Diaminobenzene dihydrochloride

Size Price ($) Quantity Availability
25g 41.72
100g 166.86
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o-Phenylenediamine dihydrochloride, 98+%

MDL
MFCD00012966
EINECS
210-418-7

Chemical Properties

Formula
C6H8N2•2HCl
Formula Weight
181.07
Form
Powder
Solubility
Slightly soluble in water; freely soluble in alcohol, chloroform or ether

Applications

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Notes

Store in cool, dry place in a tightly sealed container. Store away from oxidizing agents, heat. Store in refrigerator.

Literature References

J H Bovaird; T T Ngo; H M Lenhoff. Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Clinical Chemistry.1982, 28 (12), 2423-2426.

G Wolters; L Kuijpers; J Kacaki; A Schuurs.Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. Journal of Clinical Pathology. 1976, 29 (10), 873-879.

GHS Hazard and Precautionary Statements

Hazard Statements: H301-H312-H332-H319-H317-H341-H351-H400-H410

Toxic if swallowed. Harmful in contact with skin. Harmful if inhaled. Causes serious eye irritation. May cause an allergic skin reaction. Suspected of causing genetic defects. Suspected of causing cancer. Very toxic to aquatic life. Very toxic to aquatic life with long lasting effects.

Precautionary Statements: P201-P261-P273-P280-P281-P301+P310-P305+P351+P338-P304+P340-P405-P501a

Obtain special instructions before use. Avoid breathing dust/fume/gas/mist/vapours/spray. Avoid release to the environment. Wear protective gloves/protective clothing/eye protection/face protection. Use personal protective equipment as required. IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Beilstein
3912045
Hazard Class
6.1
Packing Group
III
Harmonized Tariff Code
2921.51
TSCA
Yes
RTECS
ST0175000

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