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TRIS-buffered saline (TBS, 20X), with 2% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein:protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate. TBS-Tween 20 is often used for washing steps.
A Molloy.; P Laochumroonvorapong. Apoptosis, but not necrosis, of infected monocytes is coupled with killing of intracellular bacillus Calmette-Guerin. . JEM. 1994, 180 ,(4), 1499-1509 .