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Laemmli SDS sample buffer, non-reducing (6X) - J60660 - Alfa Aesar

J60660 Laemmli SDS sample buffer, non-reducing (6X)

CAS Number

Size Price ($) Quantity Availability
25ml 56.24
50ml 88.37
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Laemmli SDS sample buffer, non-reducing (6X)

Chemical Properties

It is soluble in water.


Laemmli SDS sample buffer, non-reducing (6X) is an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Composition of this buffer is similar to the reducing buffer minus mercaptoethanol. This product is ideal for polyacrylamide protein gel analysis.


Contains 375mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, and 0.03% bromophenol blue.

Literature References

Bolt and Mahoney. High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate- polyacrylamide, gel electrophoresis. Analytical Biochemistry.  1997, 247, 185-192.

SE Lyons.; ME Bruck.; EJ Bowie.; D Ginsburg. Impaired intracellular transport produced by a subset of type IIA von Willebrand disease mutations. Journal of Biological chemistry. 1992, 267, 4424-4430.

GHS Hazard and Precautionary Statements

Hazard Statements: H319

Causes serious eye irritation.

Precautionary Statements: P280-P264-P305+P351+P338-P337+P313

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Other References

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