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631-61-8 - Ammonium acetate, 5M aq. soln. - J60688 - Alfa Aesar

J60688 Ammonium acetate, 5M aq. soln.

CAS Number

Size Price ($) Quantity Availability
50ml 27.50
100ml 44.00
250ml 69.00
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Ammonium acetate, 5M aq. soln.


Chemical Properties

Formula Weight
Soluble in alcohol (freely), acetone (slightly), water (148 g/100ml) at 4°C, and water (7.89 g/100ml) at 15°C.


Ammonium acetate is used for routine precipitation of nucleic acids, and is especially useful for reducing the coprecipitation of unwanted dNTPs and contaminating oligosaccharides. It is used as an important reagent for studying molecular biology, biological buffers, reagents and DNA and RNA purification. It is a popular buffer for mobile phases for HPLC with ESLD detection, for ESI mass spectrometry of proteins and other molecules, and has been used to replace cell buffers with non-volitile salts. Ammonium acetate is also used in protein studies and protein preparation. It can be used in the protein purification steps of dialysis to remove contaminants through diffusion and, when combined with distilled water, as a protein precipitating agent. In organic chemistry, ammonium acetate solution is useful as a catalyst in the Knoevenagel condensation and as a source of ammonia in the Borch reaction. Additionally, it is occasionally used commercially as a biodegradable de-icing agent and as an additive in food as an acidity regulator.


Store only at -20°C in a freezer without a defrost cycle.

Literature References

AM Maxam.; W Gilbert. A new method for sequencing DNA. PNAS. 1977, 74,(2), 560-564.

MG Ikonomou.; AT Blades.; P Kebarle. Electrospray mass spectrometry of methanol and water solutions suppression of electric discharge with SF6 gas. Journal of the American Society for Mass Spectrometry. 1991, 2,(6), 497-505.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code


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