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Catalyzes the hydrolysis of peptidoglycans found in bacterial cell walls. Lysozyme catalyzes is utilized for the hydrolysis of peptidoglycans found in bacterial cell walls. It is used as a precursor in the production of spheroplasts. It is also used as a preservative against many food spoiling microorganisms. It is used in skin care in order to cure and prevent acne and bed sores, dental and oral conditions. It is used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. Further, it is used in non-pasteurized beer.
Minimum ≥20,000 units/mg protein. One unit is the amount of enzyme causing a decrease in absorbance at 450nm of 0.001 per minute at 25°C and pH 6.2 with Micrococcous Lysodeikticus as a substrate in a 2.6 mL reaction mixture (1 cm light path).
For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/ml) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should retain activity for at least one month when stored between 2–8°C.
Bayramoglu, G.; Ozalp, V. C.; Yilmaz, M.; Guler, U.; Salih, B.; Arica, M. Y. Lysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance (QCM)-based determination of lysozyme from chicken egg white. Microporous Mesoporous Mater. 2015, 207, 95-104.
Gao, R.; Zhang, L.; Hao, Y.; Cui, X.; Liu, D.; Zhang, M.; Tang, Y. Novel polydopamine imprinting layers coated magnetic carbon nanotubes for specific separation of lysozyme from egg white. Talanta 2015, 144, 1125-1132.
Hazard Statements: H334
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statements: P261-P285-P304+P341-P342+P311-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. In case of inadequate ventilation wear respiratory protection. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Dispose of contents/ container to an approved waste disposal plant