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J60766 NP-40 lysis buffer

CAS Number

Stock No. Size Price ($) Quantity Availability
J60766-AK 250ml 41.80
J60766-AP 500ml 66.80
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NP-40 lysis buffer

Chemical Properties

Storage & Sensitivity
Keep Cold.
It is soluble in water.


NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions.


This buffer contains: 50mM Tris-HCl (pH 7.4), 150mM NaCl, 1%NP-40 and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literature References

M Herrmann.; HM Lorenz.; R Voll.; M Grünke. A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res. 1994, 22,(24), 5506-5507.< p>

K Guo.; J Wang.; V Andres.; RC Smith. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation. Mol. Cell. Biol. 1995, 15,(7), 3823-3829.< p>

Other References

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