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J60808 3,3',5,5'-Tetramethylbenzidine aq. soln.

CAS Number
54827-17-7
Synonyms
TMB soluble reagent, aq. soln.

Stock No. Size Price ($) Quantity Availability
J60808-AE 100ml 123.00
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3,3',5,5'-Tetramethylbenzidine aq. soln.

MDL
MFCD00007748
EINECS
259-364-6

Chemical Properties

Formula
C16H20N2
Formula Weight
240.35
Form
Liquid
Storage & Sensitivity
Keep Cold.
Solubility
Slightly soluble. <0.1 g/100 mL at 20°C.

Applications

3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays. 3,3?,5,5?-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood assay of hemoglobin assay of peroxidases.

Notes

Contains TMB in an aqueous buffer, pH 3.6 ± 0.2, with 0.03% hydrogen peroxide. The TMB product is soluble after conversion

Literature References

J C Standefer,; D Vanderjagt. Use of tetramethylbenzidine in plasma hemoglobin assay.. Clinical Chemistry. 1977, 23 (4), 749-751.

R C Lijana,; M C Williams. Tetramethylbenzidine--a substitute for benzidine in hemoglobin analysis.. Journal of Laboratory and Clinical Medicine. 1979, 94 (2), 266-276.

Other References

Beilstein
2808541
Harmonized Tariff Code
2921.59
TSCA
Yes

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