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CHAPS lysis buffe is used for cell lysis and protein extraction. CHAPS lysis buffer ideal for general cell-tissue lysis and subcellular orgnalle or molecular fractionation. CHAPS is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies. The addition of calcium chloride to the CHAPS reagent preserves calcium dependent protein-protein interaction, whereas the addition of EDTA abrogates the interaction. Chaps can be used with tissues as well as culture cells, effective as a stand alone reagent for cell lysis and protein extraction as well as for immunoprecipitation,
Honglai Zhang,; Jin Koo Kim,; Chris A. Edwards,; Zhaohui Xu,; Russell Taichman,; Cun-Yu Wang. Clusterin inhibits apoptosis by interacting with activated Bax. Nature Cell Biology . 2005, 7 909 - 915.
Franz Oswald,; Michael Winkler,; Ying Cao,; Kathy Astrahantseff,; Soizic Bourteele,; Walter Knöchel,; Tilman Borggrefe. RBP-Jκ/SHARP Recruits CtIP/CtBP Corepressors To Silence Notch Target Genes. Mol. Cell. Biol. 2005 , 25 (23), 10379-10390.