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PBS with EDTA is also used to disengage attached and clumped cells. PBS-EDTA is used as a buffer in procedures generating dendritic cells from monocytes. PBS+EDTA to get better cell sorting. PBS/EDTA may also be used directly without addition of trypsine for very gentle cell dissociation. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry. PBS can be used as a diluent in methods to dry biomolecules.
Patterson,; Margaret F.,; Quinn, Michele, Simpson, Ryan; Gilmour, Arthur. Sensitivity of Vegetative Pathogens to High Hydrostatic Pressure Treatment in Phosphate-Buffered Saline and Foods. Journal of Food Protection. 1995 , 5473-578.
Dulbecco, R. et al. Plaque formation and isolation of pure lines with poliomyelitis viruses.. J. Exp. Med.1954, 99(2), 167-182.
Hazard Statements: H333-H373
May be harmful if inhaled. May cause damage to organs through prolonged or repeated exposure.
Precautionary Statements: P260-P304+P312a-P314-P501a
Do not breathe dust/fume/gas/mist/vapours/spray. IF INHALED: Call a POISON CENTER/doctor if you feel unwell. Get medical advice/attention if you feel unwell. Dispose of contents/container in accordance with local/regional/national/international regulations.