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J60902 NP-40 lysis buffer, low salt

CAS Number
Synonyms

Stock No. Size Price ($) Quantity Availability
J60902-AK 250ml 41.10
J60902-AP 500ml 65.40
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NP-40 lysis buffer, low salt

Chemical Properties

Form
Liquid
Storage & Sensitivity
Keep Cold.
Solubility
Fully misicible in water.

Applications

Lysis buffer for extracting proteins from cells. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA. This lysis buffer can be used to prepare cell lysates for western blots or immunoprecipitation. It can also be used as a wash buffer for immunoprecipitation reactions.

Notes

This buffer contains: 50mM Tris-HCl (pH 7.4), 1% NP-40, and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literature References

K Guo, J Wang,; V Andrés,; R C Smith and K Walsh. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation.. Mol. Cell. Biol.1995, 15 (7), 3823-3829.

J L Taupin,; Q Tian,; N Kedersha,; M Robertson,; P Anderson The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death.. PNAS. year of publication , 92 (5) , 1629-1633.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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