Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

NP-40 lysis buffer, low salt - J60902 - Alfa Aesar

J60902 NP-40 lysis buffer, low salt

CAS Number

Size Price ($) Quantity Availability
250ml 39.80
500ml 63.60
Add to Cart Add to Quote Request View Item

NP-40 lysis buffer, low salt

Chemical Properties

Fully misicible in water.


Lysis buffer for extracting proteins from cells. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA. This lysis buffer can be used to prepare cell lysates for western blots or immunoprecipitation. It can also be used as a wash buffer for immunoprecipitation reactions.


This buffer contains: 50mM Tris-HCl (pH 7.4), 1% NP-40, and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literature References

K Guo, J Wang,; V Andrés,; R C Smith and K Walsh. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation.. Mol. Cell. Biol.1995, 15 (7), 3823-3829.

J L Taupin,; Q Tian,; N Kedersha,; M Robertson,; P Anderson The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death.. PNAS. year of publication , 92 (5) , 1629-1633.

Other References

Harmonized Tariff Code


  • H31447

    Ethyl thiooxamate, 95%
  • J66059

    Antifreeze Polypeptide (AFP) (HPLC-6), Winter Flounder
  • A10875

    3,5-Dibromopyridine, 98+%
  • 31484

    Al-23 Insulating Tube, Both Ends Open (Thin Wall);OD (mm), 1.5;ID (mm), 1
  • L00873

    3-Bromo-2,4,6-trimethylaniline, 98+%

Recently Viewed


Life Science

Metals & Materials


Analytical & Labware