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J60902 NP-40 lysis buffer, low salt

CAS Number

Stock No. Size Price ($) Quantity Availability
J60902-AK 250ml 41.10
J60902-AP 500ml 65.40
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NP-40 lysis buffer, low salt

Chemical Properties

Storage & Sensitivity
Keep Cold.
Fully misicible in water.


Lysis buffer for extracting proteins from cells. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA. This lysis buffer can be used to prepare cell lysates for western blots or immunoprecipitation. It can also be used as a wash buffer for immunoprecipitation reactions.


This buffer contains: 50mM Tris-HCl (pH 7.4), 1% NP-40, and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literature References

K Guo, J Wang,; V Andrés,; R C Smith and K Walsh. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation.. Mol. Cell. Biol.1995, 15 (7), 3823-3829.

J L Taupin,; Q Tian,; N Kedersha,; M Robertson,; P Anderson The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death.. PNAS. year of publication , 92 (5) , 1629-1633.

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