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69-53-4 - Ampicillin - J60977 - Alfa Aesar

J60977 Ampicillin

CAS Number
69-53-4
Synonyms

Size Price ($) Quantity Availability
5g 37.30
25g 175.00
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Ampicillin

MDL
MFCD00005175
EINECS
200-709-7

Chemical Properties

Formula
C16H19N3O4S
Formula Weight
349.40
Form
Powder
Melting point
208° dec.
Solubility
In 1 M NH4OH, 50 mg/ml

Applications

Ampicillin is used as asemi-synthetic antibiotic which has antibacterial activity structurally related to penicillin. It is widely used selection reagent for transformed cells expressing β-lactamase. It is also used to prevent and treat a number of bacterial infections. It may also be used to prevent group B streptococcal infection in newborns.

Notes

Stock solution:

An ampicillin stock solution can be prepared at a concentration of 100 mg/mL and should be stored at -20⁰C.

  1. Add 1g of ampicillin to 10mL of dH2O
  2. Sterilize the solution using a 0.22μm filter
  3. Store solution in different aliquots at -20⁰C

LB-ampicillin agar preparation:

  1. Dissolve the following in 500 mL dH2O:
    1. 5g tryptone
    2. 2.5g yeast extract
    3. 5.0g NaCl
    4. 7.5g agar
    5. 25mg ampicillin

    OR

    1. 20g pre-mixed LB agar powder
    2. 25mg ampicillin
  2. Boil solution on stirring hot plate for 1 - 2 min.
  3. Autoclave for 20 minutes and let cool to 50-60⁰C.
  4. Pour approximately 10mL of molten LB agar into each plate.
  5. Allow plates to solidify for approx. 20 min

Research Use only: Not intended for animal or human diagnostic or therapeutic use.

Literature References

Zhao Z., Baldo BA., O'Brien RM., Plomley RF. Reaction with, and fine structural recognition of polyamines by human IgE antibodies. Molecular immunology . 2000, 37,(5), 233-240.

Harmoinen J., Vaali K., Koski P., Syrjänen K., Laitinen O., Lindevall K., Westermarck E. Enzymic degradation of a beta-lactam antibiotic, ampicillin, in the gut: a novel treatment modality. The Journal of antimicrobial chemotherapy . 2003, 51,(2), 361-365.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H334-H317-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction. May cause respiratory irritation.

Precautionary Statements: P260-P201-P280g-P304+P340-P405-P501a

Do not breathe dust/fume/gas/mist/vapours/spray. Obtain special instructions before use. Wear protective gloves. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,586
Beilstein
1090925
Harmonized Tariff Code
2941.10
TSCA
No
RTECS
XH8350000

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