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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3 - J60992 - Alfa Aesar

J60992 TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3

CAS Number

Size Price ($) Quantity Availability
500ml 61.20
1L 116.00
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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3

Chemical Properties

It is soluble in water.


Tris Tricine Gel Electrophoresis is routinely used for the separation of small proteins and peptides with a molecular weight of I to 100 kDa. It is used for protein analysis. This type of gel is perfectly suited for the separation and identification of proteins in samples containing only a few different proteins to samples containing complex mixtures (e.g. from cell extracts).


1M Tris base, 1M Tricine, 1% SDS, pH 8.3.

Literature References

Dunker A K & Rueckert R R. Observations on molecular weight determinations on polyacrylamide gel. J. Biol. Chem. 1969, 244,18), 5074-5080.

Laemmli, U. K. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature. 1970, 227, 680 - 685.

Other References

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