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Tris Tricine Gel Electrophoresis is routinely used for the separation of small proteins and peptides with a molecular weight of I to 100 kDa. It is used for protein analysis. This type of gel is perfectly suited for the separation and identification of proteins in samples containing only a few different proteins to samples containing complex mixtures (e.g. from cell extracts).
Dunker A K & Rueckert R R. Observations on molecular weight determinations on polyacrylamide gel. J. Biol. Chem. 1969, 244,18), 5074-5080.
Laemmli, U. K. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature. 1970, 227, 680 - 685.
Hazard Statements: H315-H319
Causes skin irritation. Causes serious eye irritation.
Precautionary Statements: P264b-P280-P302+P352-P305+P351+P338-P332+P313-P337+P313-P362
Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse.