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9001-78-9 - Alkaline Phosphatase, calf intestine, EIA Grade - EC 3.1.3.1 - CIAP - J61037 - Alfa Aesar

J61037 Alkaline Phosphatase, calf intestine, EIA Grade

CAS Number
9001-78-9
Synonyms
EC 3.1.3.1
CIAP

Size Price ($) Quantity Availability
1mg 122.00
5mg 514.00
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Alkaline Phosphatase, calf intestine, EIA Grade

MDL
MFCD00131849
EINECS
232-631-4

Chemical Properties

Form
Liquid, 140 kDa
Solubility
It is soluble in water.

Applications

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Notes

Minimum 3,000 units per mg protein. One unit hydrolyzes 1 micromole of p-nitrophenol phosphate per minute at 37 degrees and pH 9.8

Literature References

C Wittmann., B Hock. Development of an ELISA for the analysis of atrazine metabolites deethylatrazine and deisopropylatrazine. J. Agric. Food Chem. 1991, 39,(6), 1194-1200.

Other References

Harmonized Tariff Code
3507.90
TSCA
Yes

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