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9003-98-9 - Deoxyribonuclease I, bovine pancreas, Purified - EC 3.1.21.1 - DNase I - J61061 - Alfa Aesar

J61061 Deoxyribonuclease I, bovine pancreas, Purified

CAS Number
9003-98-9
Synonyms
EC 3.1.21.1
DNase I

Size Price ($) Quantity Availability
5mg 44.96
20mg 102.40
100mg 462.47
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Deoxyribonuclease I, bovine pancreas, Purified

MDL
MFCD00130918
EINECS
232-667-0

Chemical Properties

Form
Lyophilized powder, 31 kDa
Solubility
It is soluble in 0.15 M NaCl (5 mg/ml).

Applications

Deoxyribonuclease I, is used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. It is used in investigating the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Notes

Minimum 2,000 units per mg protein. One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0

Literature References

PA Price., TY Liu., WH Stein., S Moore. Properties of chromatographically purified bovine pancreatic deoxyribonuclease. The Journal of Biological Chemistry. 1969, 244, 917-923.

J Salnikow., S Moore., WH Stein. Comparison of the multiple forms of bovine pancreatic deoxyribonuclease. The Journal of Biological Chemistry. 1970, 245, 5685-5690.

Other References

Merck
14,2906
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
RF0750000

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