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77-86-1 - TRIS, 1.0M buffer soln., pH 8.6 - J61066 - Alfa Aesar

J61066 TRIS, 1.0M buffer soln., pH 8.6

CAS Number
77-86-1
Synonyms

Size Price ($) Quantity Availability
1L 48.20
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TRIS, 1.0M buffer soln., pH 8.6

MDL
MFCD00004679

Chemical Properties

Form
Liquid
Solubility
Fully miscible in water. Soluble in ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.

Applications

TRIS is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. It has a pKa of 8.06 and is very useful in the molecular biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Notes

Stable under recommended storage conditions. Incompatible with oxidizing agents.

Literature References

Steven P.Fling; Dale S.Gregerson. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Analytical Biochemistry. 1986, 155,(1), 83-88.

 

R.T.Irvin; T.J.MacAlister; J.W.Costerton. Tris(hydroxymethyl)aminomethane buffer modification of Escherichia coli outer membrane permeability. J Bacteriol. 1981, 145,(3), 1397-1403.

Other References

Harmonized Tariff Code
2922.19
TSCA
Yes

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