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BSA blocking buffer, 5% in PBS - J61089 - Alfa Aesar

J61089 BSA blocking buffer, 5% in PBS

CAS Number

Size Price ($) Quantity Availability
250ml 72.31
500ml 94.40
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BSA blocking buffer, 5% in PBS

Chemical Properties

Slightly miscible with water and methanol. Immiscible with diethyl ether and n-octanol.


BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.


5% Bovine serum albumin in Phosphate-buffered saline, pH 7.4Store in a cool place. Incompatible with metals and acids.

Literature References

Wittmann, K.; Dietl, S.; Ludwig, N.; Berberich, O.; Hoefner, C.; Storck, K.; Blunk, T.; Bauer-Kreisel, P. Engineering Vascularized Adipose Tissue Using the Stromal-Vascular Fraction and Fibrin Hydrogels. Tissue Eng. Part A 2015, 21 (7-8), 1343-1353.

Reichard, A. C.; Cheemarla, N. R.; Bigley, N. J. SOCS1/3 Expression Levels in HSV-1-Infected, Cytokine-Polarized and -Unpolarized Macrophages. J. Interferon Cytokine Res. 2015, 35 (1), 32-41.

Other References

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