Citrate, 0.5M buffer soln., pH 4.0 may be used for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity as a calcium chelator.
JM Chirgwin.; AE Przybyla.; RJ MacDonald. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18,(24), 5294-5299.
PJ Elving.; JM Markowitz.; I Rosenthal. Preparation of buffer systems of constant ionic strength. Analytical Chemistry. 1956, 28,(7), 1179-1180.
Hazard Statements: H318
Causes serious eye damage.
Precautionary Statements: P280i-P305+P351+P338-P310a
Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor