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6132-04-3 - Citrate, 0.5M buffer soln., pH 4.0 - J61249 - Alfa Aesar

J61249 Citrate, 0.5M buffer soln., pH 4.0

CAS Number

Size Price ($) Quantity Availability
250ml 55.70
500ml 87.50
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Citrate, 0.5M buffer soln., pH 4.0


Chemical Properties

It is soluble in water.


Citrate, 0.5M buffer soln., pH 4.0 may be used for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity as a calcium chelator.


Store at room temperature.

Literature References

JM Chirgwin.; AE Przybyla.; RJ MacDonald. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18,(24), 5294-5299.

PJ Elving.; JM Markowitz.; I Rosenthal. Preparation of buffer systems of constant ionic strength. Analytical Chemistry. 1956, 28,(7), 1179-1180.

GHS Hazard and Precautionary Statements

Hazard Statements: H318

Causes serious eye damage.

Precautionary Statements: P280i-P305+P351+P338-P310a

Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor

Other References

Harmonized Tariff Code


  • J60569

    CAPS, 0.5M buffer soln., pH 10.0
  • J60712

    HEPES, 1.0M buffer soln., pH 7.5
  • J60964

    Acetate, 1M buffer soln., pH, 5.0
  • J63791

    Sodium phosphate, 0.5M buffer soln., pH 7.0
  • J63637

    Sodium borate, 0.5M buffer soln., pH 9.0

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