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54827-17-7 - 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity - TMB soluble reagent, high sensitivity - J61325 - Alfa Aesar

J61325 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

CAS Number
54827-17-7
Synonyms
TMB soluble reagent, high sensitivity

Size Price ($) Quantity Availability
125ml 112.50
500ml 183.00
1000ml 288.00
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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

MDL
MFCD00007748
EINECS
259-364-6

Chemical Properties

Formula
C16H20N2
Formula Weight
240.35
Form
Liquid
Solubility
Insoluble in water. Soluble in organic solvents such as DMSO, 96 % Ethanol, Acetone, Chloroform, Toluene.

Applications

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Notes

The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.

Literature References

T Giersch. A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiter plate and dipstick format. J. Agric. Food Chem. 1993, 41, (6), 1006-1011.

AR Johnsen.; OS Jacobsen. A quick and sensitive method for the quantification of peroxidase activity of organic surface soil from forests. Soil Biology and Biochemistry. 2008, 40, (3), 814-821.

GHS Hazard and Precautionary Statements

Hazard Statements: H312-H332-H370

Harmful in contact with skin. Harmful if inhaled. Causes damage to organs.

Precautionary Statements: P260-P280h-P304+P340-P307+P311a-P405-P501a

Do not breathe dust/fume/gas/mist/vapours/spray. Wear protective gloves/clothing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF exposed: Call a POISON CENTER/doctor. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Beilstein
2808541
Hazard Class
6.1
Packing Group
III
Harmonized Tariff Code
2921.59
TSCA
Yes

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