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TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
T Giersch. A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiter plate and dipstick format. J. Agric. Food Chem. 1993, 41, (6), 1006-1011.
AR Johnsen.; OS Jacobsen. A quick and sensitive method for the quantification of peroxidase activity of organic surface soil from forests. Soil Biology and Biochemistry. 2008, 40, (3), 814-821.
Hazard Statements: H312-H332-H370
Harmful in contact with skin. Harmful if inhaled. Causes damage to organs.
Precautionary Statements: P260-P280h-P304+P340-P307+P311a-P405-P501a
Do not breathe dust/fume/gas/mist/vapours/spray. Wear protective gloves/clothing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF exposed: Call a POISON CENTER/doctor. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.