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J61325 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

CAS Number
TMB soluble reagent, high sensitivity

Stock No. Size Price ($) Quantity Availability
J61325-=E 125ml 167.00
J61325-AP 500ml 267.00
J61325-AU 1000ml 406.00
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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity


Chemical Properties

Formula Weight
Storage & Sensitivity
Keep Cold.
Insoluble in water. Soluble in organic solvents such as DMSO, 96 % Ethanol, Acetone, Chloroform, Toluene.


TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.


The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.

Literature References

T Giersch. A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiter plate and dipstick format. J. Agric. Food Chem. 1993, 41, (6), 1006-1011.

AR Johnsen.; OS Jacobsen. A quick and sensitive method for the quantification of peroxidase activity of organic surface soil from forests. Soil Biology and Biochemistry. 2008, 40, (3), 814-821.

GHS Hazard and Precautionary Statements

Hazard Statements: H226-H302+H312-H331-H336-H370

Flammable liquid and vapour. Harmful if swallowed. Harmful in contact with skin. Toxic if inhaled. May cause drowsiness or dizziness. Causes damage to organs.

Precautionary Statements: P210-P233-P235-P240-P241-P242-P243-P260-P264b-P270-P271-P280-P301+P312-P303+P361+P353-P304+P340-P307+P311-P311-P312-P330-P363-P370+P378q-P501c

Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Keep container tightly closed. Keep cool. Ground/bond container and receiving equipment. Use explosion-proof electrical/ventilating/lighting/ equipment. Use only non-sparking tools. Take precautionary measures against static discharge. Do not breathe dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF exposed: Call a POISON CENTER or doctor/physician. Rinse mouth. Wash contaminated clothing before reuse. In case of fire: Use CO2, dry chemical, or foam Dispose of contents/ container to an approved waste disposal plant

Other References

Hazard Class
Packing Group
Harmonized Tariff Code


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