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Laemmli SDS sample buffer, reducing (6X) - J61337 - Alfa Aesar

J61337 Laemmli SDS sample buffer, reducing (6X)

CAS Number

Size Price ($) Quantity Availability
25ml 56.24
50ml 88.37
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Laemmli SDS sample buffer, reducing (6X)

Chemical Properties

Miscible with water.


For protein sample preparation to be used in the Laemmli SDS-PAGE systemLaemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Further, it is used for polyacrylamide protein gel analysis.


Contains 375mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% beta-mercaptoethanol, 0.03% bromophenol blueStore in a cool place. Incompatible with oxidizing agents.

Literature References

Lee, G. S.; Kappler, K.; Porter, C. J. H.; Scanlon, M. J.; Nicolazzo, J. A. Fatty Acid Binding Proteins Expressed at the Human Blood-Brain Barrier Bind Drugs in an Isoform-Specific Manner. Pharm. Res. 2015, 32 (10), 3432-3446.

Perot, E.; Enjolras, N.; Le Quellec, S.; Indalecio, A.; Girard, J.; Negrier, C.; Dargaud, Y. Expression and characterization of a novel human recombinant factor IX molecule with enhanced in vitro and in vivo clotting activity. Thromb. Res. 2015, 135 (5), 1017-1024.

GHS Hazard and Precautionary Statements

Hazard Statements: H318-H315-H302-H312-H412

Causes serious eye damage. Causes skin irritation. Harmful if swallowed. Harmful in contact with skin. Harmful to aquatic life with long lasting effects.

Precautionary Statements: P273-P280-P305+P351+P338-P310-P362-P301+P312-P312-P363-P321-P501a

Avoid release to the environment. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Wash contaminated clothing before reuse. Specific treatment (see label). Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code


  • J60660

    Laemmli SDS sample buffer, non-reducing (6X)
  • J60015

    Laemmli SDS sample buffer, reducing (4X)
  • J60580

    CHAPS lysis buffer
  • J61016

    Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.5%
  • J61529

    RIPA buffer with EDTA

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