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J61373 Pyruvate Kinase, rabbit muscle

CAS Number
9001-59-6
Synonyms
Phosphoenolpyruvate kinase
Phosphoenol transphosphorylase

Stock No. Size Price ($) Quantity Availability
J61373-!V 50kilounits 411.00
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Pyruvate Kinase, rabbit muscle

MDL
MFCD00081946
EINECS
232-616-2

Chemical Properties

Form
Lyophilized powder
Storage & Sensitivity
Store at -20°C.
Solubility
Soluble in water ( 1mg/ml).

Applications

Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.

Notes

Minimum 375 units per mg solid; 500 units per mg protein. One unit liberates 1 micromole pyruvate per minute from PEP in the presence of ADP at 30 C and pH 7.6.

Literature References

Takanori Kigawa; Takashi Yabuki; Natsuko Matsuda; Takayoshi Matsuda; Rie Nakajima; Akiko Tanaka; Shigeyuki Yokoyama. Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. Journal of Structural and Functional Genomics. 2004, 5,(1-2), 63-68.

 

I.Mor; R.Carlessi; T.Ast; E.Feinstein; A.Kimchi. Death-associated protein kinase increases glycolytic rate through binding and activation of pyruvate kinase. Oncogene. 2012, 31,(6), 683-693.

Other References

Harmonized Tariff Code
3507.90
TSCA
Yes

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