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J61462 Luminol and oxidizing solutions (each bottle)

CAS Number

Stock No. Size Price ($) Quantity Availability
J61462-AK 250ml 106.00
J61462-AP 500ml 179.00
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Luminol and oxidizing solutions (each bottle)

Chemical Properties

Storage & Sensitivity
Light Sensitive. Keep Cold.
It is soluble in most polar organic solvents but is insoluble in water.


Used by biologists in cellular assays for the detection of copper, iron, and cyanides, as well as of specific proteins by western blot. For chemiluminecent HRP-based detection of western blotting systems. Microestimation of glucose and glucose oxidase using enzyme-induced chemiluminescence6,7 - Inhibitor of poly ADP Ribose Synthase8 - Demonstrations of the phenomenon of chemiluminescence, using different sensitizers to produce different colors of light.


Mix equal amounts of luminol and oxidizing solution, then incubate the membrane for 1 min. Expose membrane to x-ray film for at least one minute. Adjust exposure as necessary.

Literature References

AL Rose.; TD Waite. Chemiluminescence of luminol in the presence of iron (II) and oxygen: oxidation mechanism and implications for its analytical use. Analytical chemistry.2001, 73, (24), 5909-5920.

WR Seitz.; DM Hercules. Quantitative study of chemiluminescence from the iodine-luminol reaction. J. Am. Chem. Soc.1974, 96, (13), 4094-4098.

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