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Luminol and oxidizing solutions (each bottle) - J61462 - Alfa Aesar

J61462 Luminol and oxidizing solutions (each bottle)

CAS Number

Size Price ($) Quantity Availability
250ml 104.03
500ml 177.16
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Luminol and oxidizing solutions (each bottle)

Chemical Properties

Light Sensitive
It is soluble in most polar organic solvents but is insoluble in water.


Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes, as it reacts with iron found in hemoglobin. It is used by biologists in cellular assays for the detection of copper, iron, and cyanides, as well as of specific proteins by western blot. For chemiluminecent HRP-based detection of western blotting systems. Microestimation of glucose and glucose oxidase using enzyme-induced chemiluminescence6,7 - Inhibitor of poly ADP Ribose Synthase8 - Demonstrations of the phenomenon of chemiluminescence, using different sensitizers to produce different colors of light.


Mix equal amounts of luminol and oxidizing solution, then incubate the membrane for 1 min. Expose membrane to x-ray film for at least one minute. Adjust exposure as necessary.

Literature References

AL Rose.; TD Waite. Chemiluminescence of luminol in the presence of iron (II) and oxygen: oxidation mechanism and implications for its analytical use. Analytical chemistry.2001, 73, (24), 5909-5920.

WR Seitz.; DM Hercules. Quantitative study of chemiluminescence from the iodine-luminol reaction. J. Am. Chem. Soc.1974, 96, (13), 4094-4098.

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