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J61544 Tween 20 blocking buffer, 1% in PBS (10X)

CAS Number

Stock No. Size Price ($) Quantity Availability
J61544-AP 500ml 41.10
J61544-K2 1L 60.60
J61544-K3 2L 114.00
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Tween 20 blocking buffer, 1% in PBS (10X)

Chemical Properties

Viscous liquid
Storage & Sensitivity
Keep Cold.
Soluble in water


Tween 20 blocking buffer solution is used as a washing agent in immunoassays, such as western blots and ELISAs. It helps to prevent non-specific antibody binding. The buffer is used for washes between each immunoreactions, to remove unbound immunological, and eventually for incubation solutions of immunoreagents to reduce unspecific background.


J61544 is 10X and the concentration of Tween-20 in this is 1%. The final concentration of Tween 20 in 1X buffer will be 0.1%.

Literature References

JL Pace.; PJ Morales.; JS Hunt.; TA Phillips. Analysis of the soluble isoforms of HLA-G. Placenta and Trophoblast.  1980, 122 181-203.

M Szı?lagyi.; VR Rivera.; D Neal.; GA Merrill.; MA Poli. Development of sensitive colorimetric capture elisas for Clostridium botulinum neurotoxin serotypes A and B. Toxicon. 2000, 38(3), 381-389.

Other References

Harmonized Tariff Code


  • J60304

    Tween 20 washing buffer, 0.5% in PBS (10X)

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