Tween 20 blocking buffer solution is used as a washing agent in immunoassays, such as western blots and ELISAs. It helps to prevent non-specific antibody binding. The buffer is used for washes between each immunoreactions, to remove unbound immunological, and eventually for incubation solutions of immunoreagents to reduce unspecific background.
JL Pace.; PJ Morales.; JS Hunt.; TA Phillips. Analysis of the soluble isoforms of HLA-G. Placenta and Trophoblast. 1980, 122 181-203.
M Szı?lagyi.; VR Rivera.; D Neal.; GA Merrill.; MA Poli. Development of sensitive colorimetric capture elisas for Clostridium botulinum neurotoxin serotypes A and B. Toxicon. 2000, 38(3), 381-389.