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J61655 BSA blocking buffer, 3% in PBS

CAS Number

Stock No. Size Price ($) Quantity Availability
J61655-AK 250ml 67.60
J61655-AP 500ml 117.00
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BSA blocking buffer, 3% in PBS

Chemical Properties

Storage & Sensitivity
Store at -20°C.
Slightly miscible with water and methanol. Immiscible with diethyl ether and n-octanol.


BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.


3% Bovine serum albumin in PBS, pH 7.4Store in a cool place. Incompatible with metals and acids.

Literature References

Kalwa, H.; Storch, U.; Demleitner, J.; Fiedler, S.; Mayer, T.; Kannler, M.; Fahlbusch, M.; Barth, H.; Smrcka, A.; Hildebrandt, F.; Gudermann, T.; Dietrich, A. Phospholipase C Epsilon (PLCepsilon) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. J. Cell. Physiol. 2015, 230 (6), 1389-1399.

Jeong, M. S.; Ahn, D. R. A microwell plate-based multiplex immunoassay for simultaneous quantitation of antibodies to infectious viruses. Analyst 2015, 140 (6), 1995-2000.

Other References

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