I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.
BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.
Kalwa, H.; Storch, U.; Demleitner, J.; Fiedler, S.; Mayer, T.; Kannler, M.; Fahlbusch, M.; Barth, H.; Smrcka, A.; Hildebrandt, F.; Gudermann, T.; Dietrich, A. Phospholipase C Epsilon (PLCepsilon) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. J. Cell. Physiol. 2015, 230 (6), 1389-1399.
Jeong, M. S.; Ahn, D. R. A microwell plate-based multiplex immunoassay for simultaneous quantitation of antibodies to infectious viruses. Analyst 2015, 140 (6), 1995-2000.