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Pepsin is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme has also been used in the preparation of F(ab)2 fragment from IgG.
TJ McDonald.; G Nilsson.; M Vagne.; M Ghatei. A gastrin releasing peptide from the porcine nonantral gastric tissue. Gut . < span>1978, 19, (2), 767-774.
J Jancarik.; SH Kim. Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. < span>1978, 24, 409-411.
Hazard Statements: H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Precautionary Statements: P284-P261-P280a-P305+P351+P338-P405-P501a
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.