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9001-75-6 - Pepsin, porcine stomach - EC 3.4.23.1 - Pepsin A - J61679 - Alfa Aesar

J61679 Pepsin, porcine stomach

CAS Number
9001-75-6
Synonyms
EC 3.4.23.1
Pepsin A

Size Price ($) Quantity Availability
1g 115.36
5g 188.49
10g 300.00
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Pepsin, porcine stomach

MDL
MFCD00081840
EINECS
232-629-3

Chemical Properties

Form
Powder
Solubility
Soluble in water and dilute aqueous buffers

Applications

Pepsin is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme has also been used in the preparation of F(ab)2 fragment from IgG.

Notes

Minimum 2,500 units/mg. One unit increases absorbance at 280nm by 0.001 per minute at 37 C and pH 2.0, measured as TCA soluble products from denatured hemoglobin

Literature References

TJ McDonald.; G Nilsson.; M Vagne.; M Ghatei. A gastrin releasing peptide from the porcine nonantral gastric tissue. Gut . < span>1978, 19, (2), 767-774.

J Jancarik.; SH Kim. Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. < span>1978, 24, 409-411.

GHS Hazard and Precautionary Statements

Hazard Statements: H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,7146
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
SC6132000

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