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39445-21-1 - Elastase, porcine pancreas aqueous suspension - E.C. 3.4.21.36 - Pancreatopeptidase E - J61753 - Alfa Aesar

J61753 Elastase, porcine pancreas aqueous suspension

CAS Number
39445-21-1
Synonyms
E.C. 3.4.21.36
Pancreatopeptidase E

Size Price ($) Quantity Availability
25mg 57.89
100mg 160.68
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Elastase, porcine pancreas aqueous suspension

MDL
MFCD00130998
EINECS
254-453-6

Chemical Properties

Form
Suspension, MW 25000
Solubility
Soluble in water.

Applications

Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.

Notes

Minimum 3 units per mg protein. One unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25 degrees and pH 8.0. 2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron filtered before use.

Literature References

RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .

J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.

GHS Hazard and Precautionary Statements

Hazard Statements: H334

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statements: P285-P261-P342+P311-P304+P341-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,3535
Harmonized Tariff Code
3507.90
TSCA
No
RTECS
TS5052000

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