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39445-21-1 - Elastase, porcine pancreas - E.C. 3.4.21.36 - Pancreatopeptidase E - J61874 - Alfa Aesar

J61874 Elastase, porcine pancreas

CAS Number
39445-21-1
Synonyms
E.C. 3.4.21.36
Pancreatopeptidase E

Size Price ($) Quantity Availability
25mg 87.96
100mg 265.74
1g 1184.50
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Elastase, porcine pancreas

MDL
MFCD00130998
EINECS
254-453-6

Chemical Properties

Form
Lyophilized powder, MW 25000
Solubility
Soluble in water or Tris buffer up to 2.5mg/ml. Avoid high salt concentrations (ie >50mM) if salt is necessary in the buffer. Unstable in solution at pH <3.5.

Applications

This enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.

Notes

Minimum 3 units per mg protein. One unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25 degrees and pH 8.0

Literature References

RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .

J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.

GHS Hazard and Precautionary Statements

Hazard Statements: H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,3535
Harmonized Tariff Code
3507.90
TSCA
No
RTECS
TS5052000

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