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J61874 Elastase, porcine pancreas

CAS Number
39445-21-1
Synonyms
E.C. 3.4.21.36
Pancreatopeptidase E

Stock No. Size Price ($) Quantity Availability
J61874-MB 25mg 90.10
J61874-MC 100mg 279.00
J61874-03 1g 1366.00
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Elastase, porcine pancreas

MDL
MFCD00130998
EINECS
254-453-6

Chemical Properties

Form
Lyophilized powder, MW 25000
Storage & Sensitivity
Keep Cold.
Solubility
Soluble in water or Tris buffer up to 2.5mg/ml. Avoid high salt concentrations (ie >50mM) if salt is necessary in the buffer. Unstable in solution at pH <3.5.

Applications

This enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.

Notes

Minimum 3 units per mg protein. One unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25 degrees and pH 8.0

Literature References

RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .

J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H317-H319-H335

Causes skin irritation. May cause an allergic skin reaction. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P264b-P271-P272-P280-P302+P352-P304+P340-P305+P351+P338-P312-P333+P313-P362-P501c

Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation or rash occurs: Get medical advice/attention. Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Merck
14,3535
Harmonized Tariff Code
3507.90
TSCA
No
RTECS
TS5052000

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