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LDS-sample buffer (4X), non-reducing - J61894 - Alfa Aesar

J61894 LDS-sample buffer (4X), non-reducing

CAS Number

Size Price ($) Quantity Availability
25ml 47.70
50ml 79.60
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LDS-sample buffer (4X), non-reducing

Chemical Properties

Not miscible in water.


LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.


This buffer contains: 988mM Tris-HCl, 2.04mM EDTA, 8% LDS, 40% glycerol, 0.88% Coomassie blue and 0.7mM phenol red at pH 8.5.

Literature References

Yi Wang; Qiaozhen Lu; Shiaw-Lin Wu; Barry L. Karger and William S. Hancock. Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies: Using LC-MS with Electron Transfer Dissociation. Anal. Chem. 2011, 83, (8), 3133-3140.

Dan Lu; Zhenping Zhu. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy. Therapeutic Antibodies. 2008, 525, 377-404.

GHS Hazard and Precautionary Statements

Hazard Statements: H319

Causes serious eye irritation.

Precautionary Statements: P280i-P264-P305+P351+P338-P337+P313

Wear eye/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Other References

Harmonized Tariff Code


  • 36665

    Citric acid monohydrate, ACS, 99.0-102.0%
  • J61883

    Blasticidin S hydrochloride, 98+%, 10mg/ml in 1M HEPES buffer soln.
  • J63615

    Laemmli SDS sample buffer, non-reducing (4X)

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