Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

RIPA buffer with EGTA - J61951 - Alfa Aesar

J61951 RIPA buffer with EGTA

CAS Number
Synonyms

Size Price ($) Quantity Availability
250ml 64.27
500ml 112.27
Add to Cart Add to Quote Request View Item

RIPA buffer with EGTA

Chemical Properties

Form
Liquid
Solubility
Fully miscible in water.

Applications

RIPA buffer with EGTA is used in general cell-tissue lysis and subcellular orgnalle or molecular fractionation, including radio Immunoprecipitation. It is used for extracting proteins from cells. It is used for nuclear membrane disruption for nuclear extracts. It helps in disrupting protein-protein interactions.

Notes

50mM Tris-HCl (pH 7.4), 150mM NaCl 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS with 1mM EGTA.

Literature References

Robert Eskes; Solange Desagher; Bruno Antonsson and Jean-Claude Martinou. Bid Induces the Oligomerization and Insertion of Bax into the Outer Mitochondrial Membrane. Mol. Cell. Biol. 2000, 20, (3), 929-935.

Ramakrishnapillai V. Omkumar; Melinda J. Kiely; Alan J. Rosenstein; Kyung-Tai Min and Mary B. Kennedy. Identification of a Phosphorylation Site for Calcium/Calmodulindependent Protein Kinase II in the NR2B Subunit of the N-Methyl-D-aspartate Receptor. Journal of Biological Chemistry. 2000, 271, 31670-31678.

GHS Hazard and Precautionary Statements

Hazard Statements: H319

Causes serious eye irritation.

Precautionary Statements: P280-P264-P305+P351+P338-P337+P313

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

Recommended

  • 46638

    Stainless Steel Tweezer with Platinum Tips, Length 130mm
  • A16654

    3,5-Dibromobenzoic acid, 98%
  • 44905

    Zinc oxide, NanoArc® ZN-2650, 50% in H<sub>2</sub>O, colloidal dispersion with nonionic dispersant
  • 41930

    Polystyrene standard, M.W. 30,000, Mw/Mn 1.06
  • H28682

    3-Benzylbenzeneboronic acid pinacol ester, 97%

Recently Viewed

Chemicals

Life Science

Metals & Materials

Catalysts

Analytical & Labware