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J61951 RIPA buffer with EGTA

CAS Number

Stock No. Size Price ($) Quantity Availability
J61951-AK 250ml 62.90
J61951-AP 500ml 110.00
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RIPA buffer with EGTA

Chemical Properties

Storage & Sensitivity
Ambient temperatures.
Fully miscible in water.


RIPA buffer with EGTA is used in general cell-tissue lysis and subcellular orgnalle or molecular fractionation, including radio Immunoprecipitation. It is used for extracting proteins from cells. It is used for nuclear membrane disruption for nuclear extracts. It helps in disrupting protein-protein interactions.


50mM Tris-HCl (pH 7.4), 150mM NaCl 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS with 1mM EGTA.

Literature References

Robert Eskes; Solange Desagher; Bruno Antonsson and Jean-Claude Martinou. Bid Induces the Oligomerization and Insertion of Bax into the Outer Mitochondrial Membrane. Mol. Cell. Biol. 2000, 20, (3), 929-935.

Ramakrishnapillai V. Omkumar; Melinda J. Kiely; Alan J. Rosenstein; Kyung-Tai Min and Mary B. Kennedy. Identification of a Phosphorylation Site for Calcium/Calmodulindependent Protein Kinase II in the NR2B Subunit of the N-Methyl-D-aspartate Receptor. Journal of Biological Chemistry. 2000, 271, 31670-31678.

GHS Hazard and Precautionary Statements

Hazard Statements: H319

Causes serious eye irritation.

Precautionary Statements: P264b-P280i-P305+P351+P338-P337+P313

Wash face, hands and any exposed skin thoroughly after handling Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

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